Abstract: The evolution of complete reproductive isolation hinges on the cumulative action of reproductive isolating barriers that can manifest throughout the life cycle of an organism. Consequently, a comprehensive and general understanding of predictable features underlying the origin and maintenance of diverging species requires assessment of the relative contributions of distinct components of reproductive isolation in diverse taxa. Here we characterize multiple interrelated isolating barriers across the life cycle of nematode sister species Caenorhabditis remanei and Caenorhabditis latens. We quantified F1 hybrid male sterility and characterized multiple phenotypic causes associated with developmental abnormalities in the germline as well as nongermline gonad and somatic tissues, uncovering a complex suite of developmental defects contributing to strong postzygotic reproductive isolation. Despite these extensive and multifarious postzygotic isolating barrier traits, we found no evidence of premating isolation in terms of positive assortative mating for species identity as assayed from inter-species transfer of fluorescent sperm under “choice” mating conditions. In contrast to other Caenorhabditis species pairs, we also found no evidence that ectopic sperm migration acts as a postmating-prezygotic barrier. The constellation of phenotypic defects in hybrids points to a polygenic or highly pleiotropic basis for hybrid dysfunction and implicates more rapid evolution of intrinsic postzygotic reproductive isolation than prezygotic isolation in these organisms.